Bhante Dhammasubho was ordained a Bhikkhu in 1989 at Wat Bovornivet Viharn by the Most Venerable H.H. Somdet Phra Nyanasamvara, the present Sangharaja of Thailand, as his preceptor. After ordination, with the blessings of his preceptor, Bhante went to practice under the guidance of Venerable Phra Ajaan Ben Thanakaro, a meditation teacher of the Thai forest tradition at Wat Doi Dhammachedi Monastery in Sakhon Nakhon. Bhante spent his first Vassa (Rains Retreat) in that monastery.
Later on, Bhante spent three Vassas with Venerable Sayadaw U Janakabhivamsa at Chanmyay Yeiktha Meditation Centre in Yangon, Myanmar.
In 1994, Bhante went to Pa Auk Tawya Meditation Centre in Mawlamyaing to learn more about the practice of Samatha-Vipassana after being introduced to it by an Australian Bhikkhu. Bhante has since spent most of his Vassas at Pa Auk Tawya.
In early 2008, Bhante was invited to Taiwan by the Taiwandipa Theravada Buddhist College in Tainan.
In 2008, Bhante founded and set up Nandaka Vihara in Bukit Mertajam and has since been the abbot of the monastery.